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Also make sure that the tip is not cracked or chipped and check the wrapper hasn't been damaged in any way. Without opening the sterile sleeve, look through the wrapper and check that the pipette is calibrated as a 'blow-out' pipette.When working with non-sterile pipettes it is a good idea to work aseptically anyway as a matter of routine in order to practice the technique. Most of our work is done with sterile pipettes and if so, the operations are done aseptically. The Procedure for Using a Serological Pipette:
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Reading the value at the bottom of the meniscus shows that 3.19 mL of liquid was delivered. Liquid was drawn up to exactly the zero mark and was then dispensed.
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This figure illustrates how to read the meniscus on a measuring pipette. Measure using the bottom of the concave surface of the liquid in pipette. A pipette is calibrated with a series of graduation lines to allow the measurement of more than one volume.TD 20oC, (or To Deliver 20oC) which indicates the pipette will deliver the measured amount of water if the temperature is 20o centigrade.A label to indicate the size and measurement graduations of the pipette such as:.Rings to indicate that this is a 'blow-out' type of pipette.Note that the markings at the top of the pipette include:.We have three types of serological (blow-out) pipettes:.Note that this type of pipette is calibrated all the way down to the tip:.In our lab, all of the measuring pipettes will be of the blow-out variety.
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(If you are not trying to deliver the full volume of the pipette, you may not have to blow these out.)
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Untitled Document The proper use of Pipettes and Pipette-aids (Pipettors)